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FRIPS:
Free radical-induced peptide sequencing
The Technique
The FRIPS technique
was developed in the Beauchamp group for the selective sequencing of
peptides. A free radical initiator, Vazo 68 from DuPont, is
coupled to the N-terminus of the peptide in question. Collisional
activation results in loss of a part of the radical initiator, leaving
a carbon centered radical. A subsequent stage of collisional
activation leaves an acetyl radical attached to the N-terminus of the
peptide. It is this radical moiety which reacts with the peptide
backbone and sidechains to fragment it.
More Information
For more information see:
Hodyss, R,; Cox, H.
A.; Beauchamp, J.L. J. Am.
Chem. Soc. 2005, 127, 12436-12437.
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